810 FIBI (Fluorescence Imitating Brightfield Imaging) for rapid, slide-free dermatopathology

Background: Fluorescence Imitating Brightfield Imaging (FIBI) is a novel alternative microscopy method that can image freshly excised, non-sectioned tissue.We examine its potential utility in dermatopathology. Methods: Five skin samples embedded paraffin blocks were superficially deparaffinized using xylene and ethanol then briefly stained with hematoxylin eosin (H&E). FIBI captured tissue surface histology images simple microscope optics color camera. Subsequently, we applied deep learning-based models to improve resemblance standard H&E coloration contrast. compared corresponding slides concordance was assessed by two dermatopathologists who numerically scored epidermal dermal structure appearance overall diagnostic utility. Results:The mean dermatopathologist scores indicate are equivalent for visualizing structures such as layers, sweat glands, collagen. In addition, of quality most specimens including non-melanoma cancer cysts. Conclusion: Images acquired comparable traditional H&E-stained slides, suggesting this rapid, inexpensive, non-destructive technique, applicable fresh specimens, conceivable processes. Applications include time-sensitive procedures settings limited resources.